Heterocyclic compounds can be divided into two categories: alicyclic heterocycles and aromatic heterocycles. Compounds whose heterocycles in the molecular skeleton cannot reflect aromaticity are called alicyclic heterocyclic compounds. Compound: 25956-17-6, is researched, Molecular C18H14N2Na2O8S2, about An optimization approach for fast, simple and accurate determination of indigo-carmine in food samples, the main research direction is indigo carmine natural deep eutectic solvent microextraction; Central composite design; Experimental modelling; Food samples; Indigo-carmine; Natural deep eutectic solvents; Spectrophotometry.Reference of Sodium 6-hydroxy-5-((2-methoxy-5-methyl-4-sulfonatophenyl)diazenyl)naphthalene-2-sulfonate.
This study report optimization of vortex-assisted natural deep eutectic solvent based liquid-phase microextraction (VA-NADES-LPME) for determination of indigo-carmine in some food samples by UV-Visible spectrophotometer. To ensure efficient extraction, nine different NADES were prepared and tested for the extraction of indigo-carmine. In order to increase extraction efficiency of indigo-carmine, the effects of VA-NADES-LPME variables and their interactions were optimized with central composite design. The optimized method exhibited a linear range between 10 and 900 ng mL-1. Limit of detection, limit of quantification and enrichment factor were determined as 3.3 ng mL-1, 10 ng mL-1 and 135-fold, resp. The applicability of the optimized method was investigated in selected food samples using the matrix-matching calibration curve. Using optimized exptl. conditions (pH of 3.2, 75μL of NADES-4, 285μL of THF, and 4 min vortexing), satisfactory recovery results were found in the range of 95.9-104.2% with 1.4-3.7% of relative standard deviation. Finally, the optimized method was economical, simple, green, requires less laborious sampling, and provides superior accuracy and precision in trace-level anal.
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Reference:
Pyrazole – Wikipedia,
Pyrazoles – an overview | ScienceDirect Topics