In the chemical reaction process, reaction time, type of solvent, can easily affect the result of the reaction, thereby determining the yield and properties of the reaction product. An updated downstream synthesis route of 20055-01-0 as follows. COA of Formula: C4H10N4O4S
To a solution of (2S)-2- [ (BENZYLOXYCARBONYL) AMINO]-6- [ (TERT- butoxycarbonyl) amino] hexanoic acid (19.02 g) and triethylamine (5.56 g) in tetrahydrofuran (200 ml) was added methyl chloroformate (4.21 ml), followed by stirring under ice-cooling for 30 minutes. To the reaction mixture was added a solution of 1-METHYL-LH- pyrazole-4,5-diamine sulfate (15.75 g) and triethylamine (15.2 g) in water (50 ml) at the same temperature. The mixture was stirred at room temperature for 30 minutes. To the reaction mixture was added chloroform (300 ml), and the layers were separated. The organic layer was washed successively with 10% aqueous citric acid solution, brine and saturated aqueous sodium hydrogencarbonate solution. The organic layer was dried over anhydrous magnesium sulfate, filtered and concentrated in vacuo to give a crude product of 1- benzyl 5-tert-butyl (lS)- {l- [ (5-amino-l-methyl-lH- pyrazol-4-yl) CARBAMOYL] PENTAMETHYLENE} biscarbamate as an oil. The crude product was used directly in the next step without further purification. To a solution of the crude product of 1-benzyl 5- tert-butyl (LS)-{1-[(5-AMINO-1-METHYL-LH-PYRAZOL-4- yl) CARBAMOYL] PENTAMETHYLENE} BISCARBAMATE IN. methanol (350 ml) was treated with 10% palladium on carbon (2.0 g) under a hydrogen atmosphere at room temperature for 6 days. After the catalyst was filtered off, the filtrate was concentrated in vacuo. The residue was triturated with ethyl acetate and dried in vacuo to give tert-butyl (5S)-5-amino-6- [ (5-amino-l-methyl-lH-pyrazol-4- yl) amino] -6-oxohexylcarbamate (12.1 g) as a solid. H-NMR (DMSO-D6) 6 1. 24-1. 40 (4H, m), 1. 36 (9H, s), 1. 70- 1.77 (2H, m), 2.88-2. 91 (2H, m), 3.51 (3H, s), 3.80-3. 82 (1H, m), 5.15 (2H, s), 6.77 (1H, br), 7.27 (1H, s), 10.05 (1H, br)
According to the analysis of related databases, 20055-01-0, the application of this compound in the production field has become more and more popular.