Synthesis and evaluation of pyrazolo[3,4-b]pyridines and its structural analogues as TNF-α and IL-6 inhibitors was written by Bharate, Sandip B.;Mahajan, Tushar R.;Gole, Yogesh R.;Nambiar, Mahesh;Matan, T. T.;Kulkarni-Almeida, Asha;Balachandran, Sarala;Junjappa, H.;Balakrishnan, Arun;Vishwakarma, Ram A.. And the article was included in Bioorganic & Medicinal Chemistry in 2008.Product Details of 3528-58-3 This article mentions the following:
In the present article, we have synthesized three different series of pyrazolo[3,4-b]pyridines and their structural analogs using novel synthetic strategy involving one-pot condensation of 5,6-dihydro-4H-pyran-3-carbaldehyde/2-formyl-3,4,6-tri-O-methyl–glucal/chromone-3-carbaldehyde with heteroaromatic amines. All synthesized compounds were evaluated for their anti-inflammatory activity against TNF-α and IL-6. Out of 28 compounds screened, 40, 51, 52 and 56 (I) exhibited promising activity against IL-6 with 60-65% inhibition at 10 μM concentration Amongst these, 51, 52 and 56 showed potent IL-6 inhibitory activity with IC50‘s of 0.2, 0.3 and 0.16 μM, resp. Compound 56 was not cytotoxic in CCK-8 cells up to the concentration of >100 μM. In the experiment, the researchers used many compounds, for example, 1-Ethyl-1H-pyrazol-5-amine (cas: 3528-58-3Product Details of 3528-58-3).
1-Ethyl-1H-pyrazol-5-amine (cas: 3528-58-3) belongs to pyrazole derivatives. Pyrazole has two ring nitrogen atoms in which N1 is pyrrolic and N2 is pyridine-like. The N1 nitrogen is not reactive but is deprotonated in the presence of a base-forming anion. The pyrazole ring is resistant to oxidation and reduction but the groups, such as alkyl and formyl attached to the ring, are oxidized to respective acids. Only electrolytic oxidation, ozonolysis, and a strong base cause ring opening.Product Details of 3528-58-3
Referemce:
Pyrazole – Wikipedia,
Pyrazoles – an overview | ScienceDirect Topics