Simultaneous determination of N-unsubstituted and N-substituted nitroazoles and criteria for their identification. II. Chromatographic separation and polarographic determination of nitropyrazoles was written by Dumanovic, D.;Maksimovic, R.;Ciric, J.;Jeremic, D.. And the article was included in Talanta in 1974.Reference of 54210-32-1 This article mentions the following:
The N-unsubstituted nitropyrazoles have an imino H atom (in contrast to the N-substituted derivatives) and react with OH-to give nitropyrazole anions. The strongly neg. shift of E1/2 for these anions allows simultaneous polarog. determination of any pair of compounds, 1 of which is an N-unsubstituted nitropyrazole and the other a corresponding N-substituted derivative Simultaneous polarog. determination of 3 compounds [3(5)-, 3- and 5-nitropyrazoles] is also possible with 0.1M NaOH as supporting electrolyte, but only when the shift ΔE1/2 between the N-substituted isomers is ≥ 100 mV. In this case, the adequate ΔE1/2 is a result of the different electron ds. of the NO2 groups of the isomers. In the mentioned medium it is possible to determine simultaneously even 4 compounds [1-, 3(5)-, 3-and 5-nitropyrazoles], because the E1/2 value of 1-nitropyrazole does not change with pH, unlike that of other nitropyrazoles. Developers for the thin-layer chromatog. separation are proposed. Some criteria are given for distinguishing between the N-unsubstituted and the corresponding N-substituted nitropyrazoles. The structures of 2 new compounds were determined Methods are recommended for the simultaneous identification and determination of the compounds appearing together in the reaction mixtures during the substitution of the imino H atom or during the rearrangements of the 1-nitropyrazoles to the N-unsubstituted derivatives In the experiment, the researchers used many compounds, for example, 1-Methyl-3-nitro-1H-pyrazole (cas: 54210-32-1Reference of 54210-32-1).
1-Methyl-3-nitro-1H-pyrazole (cas: 54210-32-1) belongs to pyrazole derivatives. The 1H-pyrazole provides an excellent means by which to provide the requisite hydrogen bond acceptor–donor motifs, whether as a monocyclic ring or as a fused indazole ring. Pyrazole the presence of this nucleus in pharmacological agents of diverse therapeutic categories such as celecoxib, a potent anti-inflammatory, the antidepressant agent fezolamide have proved the pharmacological potential of the pyrazole moiety. Reference of 54210-32-1
Referemce:
Pyrazole – Wikipedia,
Pyrazoles – an overview | ScienceDirect Topics